normal human lung fibroblast cell line imr90  (ATCC)

Journal: PLoS ONE

Article Title: Antitumor Activity of a 5-Hydroxy-1 H -Pyrrol-2-(5 H )-One-Based Synthetic Small Molecule In Vitro and In Vivo

doi: 10.1371/journal.pone.0128928

Figure Lengend Snippet: (A) The chemical structure of 1d . (B) Cells were treated with 1d at 5 or 10 μg/ml or with DMSO (control) for 48 h and were analyzed using the MTT assay. Data are shown as the means of 2 independent experiments ± SEM. (C) The indicated cells were treated with 1d (1 μg/ml) or DMSO for the indicated days and stained with crystal violet. (D) HCT116 and IMR90 cells were treated with a serial diluted 1d for 24 h (left) and 48 h (right) and were analyzed using the MTT assay.

Article Snippet: Human colorectal carcinoma cell line HCT116, cervix adenocarcinoma cell line HeLa, osteosarcoma cell line U2OS, non-small cell lung cancer cell line H1299, liver hepatocellular carcinoma cell line HepG2 and normal human lung fibroblasts cell line IMR90 were obtained from the American Type Culture Collection (ATCC).

Techniques: Control, MTT Assay, Staining

Journal: PLoS ONE

Article Title: Antitumor Activity of a 5-Hydroxy-1 H -Pyrrol-2-(5 H )-One-Based Synthetic Small Molecule In Vitro and In Vivo

doi: 10.1371/journal.pone.0128928

Figure Lengend Snippet: (A-C) HCT116 cells treated with control (DMSO), Dox or 1d were analyzed using Western blot using the indicated antibodies (A and C) or were subjected to real-time PCR analysis (B). Data are the means of 2 independent experiments ± SEM. *, p<0.05; **, p<0.005; ***, p<0.001. (D and E) HCT116, H1299, U2OS and IMR90 cells treated with DMSO, Dox or 1d were immunostained followed by fluorescence microscopy (D) or were analyzed using Western blot using the indicated antibodies (E).

Article Snippet: Human colorectal carcinoma cell line HCT116, cervix adenocarcinoma cell line HeLa, osteosarcoma cell line U2OS, non-small cell lung cancer cell line H1299, liver hepatocellular carcinoma cell line HepG2 and normal human lung fibroblasts cell line IMR90 were obtained from the American Type Culture Collection (ATCC).

Techniques: Control, Western Blot, Real-time Polymerase Chain Reaction, Fluorescence, Microscopy

Journal: The Journal of Cell Biology

Article Title: A primate virus generates transformed human cells by fusion

doi: 10.1083/jcb.200507069

Figure Lengend Snippet: Spontaneous fusion produces proliferating hybrids. (A) IMR90 cells (I0) resistant to puromycin (I0 P ) or I0 cells transformed with E1A and resistant to hygromycin (IE H ) were cultured as indicated and stained with crystal violet to visualize the cells. (B) Co-culture of I0 P and IE H cells results in cells resistant to both drugs. Cells were plated together, treated with PEG or left untreated, cultured for 20 d, and visualized as in A. (C and D) IE H cells fuse to each other but not to themselves. I0 P and IE H cells were dyed and cultured as indicated for 16 h and visualized by fluorescence microscopy. Heterokaryons (indicated by arrows in C and scored in D) contained both green and blue dyes and at least one green and one blue nucleus. (E) Tissue culture medium from IE H cells induces cell fusion. Tissue culture medium conditioned by either I0 P or IE H cells for 16 h was tested in the fusion assay (see Materials and methods). All experiments were performed at least three times. The data in D and E are from three independent experiments, and the error bars indicate SD.

Article Snippet: Normal human diploid embryonic lung fibroblast cell line IMR90 and normal human diploid skin fibroblast cell lines Detroit 551, HSF43, and Hs68, as well as transformed monkey cell lines COS-1 and CMMT cell lines were obtained from American Type Culture Collection and cultured in 90% DME and 10% FBS and in the absence of antibiotics.

Techniques: Transformation Assay, Cell Culture, Staining, Co-Culture Assay, Fluorescence, Microscopy, Single Vesicle Fusion Assay